The second Challenge panel becomes available only when the first Challenge panel is complete.
Panel 2 Key
| B1 | B2 | B3 | B4 | B5 | B6 | B7 |
| B8 | B9 | B10 | B11 | B12 | B13 | B14 |
| B15 | B16 | B17 | B18 | B19 | B20 | B21 |
| B22 | B23 | B24 | B25 | B26 | B27 | B28 |
| B29 | B30 | B31 | B32 | B33 | B34 | B35 |
Panel 2 Details
| Position | Challenge | Reward |
|---|---|---|
| B1 |  Place first in Smash Run final battles with 5 different characters. |
Power: Lv. 2 Spinning Blades |
| B2 | Get a max combo of 40 or more in Training. | Equipment: Beam Sword Agility Badge |
| B3 |  Unlock all playable fighters. |
Music: Menu 2 (Melee) |
| B4 |  Create 3 custom characters in Custom. |
Equipment: Sprinter Agility Badge |
| B5 | Destroy 1,000 blocks in Trophy Rush. | Special: Close Combat (Ike) |
| B6 | Get 100 KOs in Smash. | Equipment: Hyper Smasher Brawn Badge |
| B7 | Collect 150 or more unique trophies. | Trophy: Redd |
| B8 | Have a total score of over 2,000,000 between all fighters in Target Blast. | Trophy: King Bob-omb |
| B9 | Get a total of 5 hits in StreetSmash. | Power: Lv. 2 Shinespark |
| B10 | Get 10 or more KOs in a single Rival Smash. | Equipment: KO Healer Protection Badge |
| B11 | Battle 3 or more times on the Living Room stage in Smash. | Trophy: Golden Retriever |
| B12 | Get 10 KOs by knocking foes into other foes in StreetSmash. | Trophy: Mugly |
| B13 | Obtain 25 types of Smash Run Powers. | Power: Lv. 2 Instant Drop |
| B14 | Clear 100-Man Smash. | Equipment: Auto-Healer Brawn Badge |
| B15 | Reveal 99% of the wall during the staff credits. | Music: Credits |
| B16 | Clear Classic with five fighters. | Trophy: Master Hand |
| B17 | Battle 3 or more times on the Find Mii stage in Smash. | Trophy: Dark Emperor |
| B18 | Win 5 Smash battles with Meta Knight. | Special: High-Speed Drill (Meta Knight) |
| B19 | Unlock the hidden character Mr. Game & Watch. | Stage: Flat Zone 2 |
| B20 |  Defeat Master Core. |
Music: Master Core |
| B21 | Get 20 or more in KOs by countering in StreetSmash. | Equipment: First Striker Agility Badge |
| B22 | Hit Sandbag 1,968 ft. or more in Home-Run Contest. | Equipment: Home-Run Bat Agility Badge |
| B23 | Get 30 or more KOs in Endless Smash. | Trophy: Fire Stingray |
| B24 | Play Smash Run 5 times. | Power: Lv. 2 Horizon Beam |
| B25 | Unlock every stage. | Trophy: Tortimer Island |
| B26 |  Play 10 collective hours of Smash. (Total gameplay × participants.) |
Trophy: Knuckle Joe |
| B27 | Win 10 Smash battles with Samus. | Headgear: Samus’s Helmet |
| B28 | Collect 15 unique special moves. | Equipment: Smash Ball Attractor Agility Badge |
| B29 | Collect 5 unique custom outfits. | Headgear: Spartan Helmet |
| B30 | Get 300 KOs in Smash. | Equipment: Critical Hitter Brawn Badge |
| B31 | Play Home-Run Contest with 15 or more fighters. | Equipment: Quick Batter Brawn Badge |
| B32 | Create 8 or more Mii Fighters in Custom. | Headgear: Top Hat |
| B33 | Collect 10 unique custom headgear items. | Outfit: Plate Armor |
| B34 | Clear All-Star on Normal. | Trophy: Medusa, Queen of the Underworld |
| B35 |  Have the game on for more than 8 hours. |
Pokémon: Meloetta |
Open all 35 of the second Challenge panel’s tiles to earn the Tutorial Pig trophy.