| Character | Level | Mode |
|---|---|---|
| Ambush Bug | Superman in Space (5-1) | Story |
| Aquaman | The Weapons Lab (5-2) | Free Play |
| Arkillo | Sinestro’s Surprise (14-2) | Story |
| Blue Beetle | Nok Approach (11-1) | Story |
| Bruce Wayne | The Bat Observatory (2-1) | Free Play |
| Catwoman | The Bat-rocket Hangar (3-1) | Free Play |
| Cheshire | The Docking Bay (5-3) | Story |
| Composite Superman | Atrocitus Attack (15-2) | Story |
| Cyborg (Superman) | The Fortress of Solitude (15-1) | Story |
| Darkest Knight Batman | Larfleeze Attack (13-3) | Story |
| Doctor Fate | Brainiac Battle (6-2) | Story |
| Doomsday | Sinestro Showdown (14-3) | Story |
| Etrigan | Watchtower Battle (3-2) | Story |
| Firestorm | Ysmault (12-2) | Story |
| Frankenstein | LEGO Paris (7-1) | Story |
| Geoff Johns | Zamaron Encounter (9-2) | Free Play |
| Goon (Croc) | Gotham Sewers (1-1) | Free Play |
| Goon (The Joker Mime) | Killer Croc (1-3) | Free Play |
| Goon (The Joker Space Henchman) | Watchtower Access (3-3) | Free Play |
| Goon (Orange Lantern) | The Bat Monster (2-3) | Story |
| Green Lantern (Guy Gardner) | Rivers of Jam (12-1) | Free Play |
| Green Lantern (John Stewart) | Larfleeze’s Vault (13-2) | Story |
| Hawkgirl | LEGO Pisa (7-3) | Free Play |
| Hawkman | Super Skydive (6-3) | Story |
| Heat Wave | The Armory (4-2) | Story |
| Kid Flash | The Underground Caverns (11-2) | Free Play |
| Kilowog | The Breach of Okaara (13-1) | Story |
| Mad Hatter | The Fun House (8-2) | Free Play |
| Manchester Black | LEGO London (7-2) | Free Play |
| Metallo | Saint Walker (10-3) | Free Play |
| Miss Martian | The Reach (10-1) | Story |
| Mr Freeze | Wayne Tower (8-3) | Story |
| Music Meister | Ace Chemicals (8-1) | Story |
| Parasite | The Mines of Qward (14-1) | Free Play |
| Poison Ivy | The Indigo Power Battery (11-3) | Story |
| Reverse Flash | Predator (9-3) | Free Play |
| Solar Suit Superman | Brainiac’s Ship (6-1) | Free Play |
| Stargirl | The Zamarons (9-1) | Story |
| Superboy | Brainiac’s Final Stand (15-3) | Story |
| Swamp Thing | Odym Jungle (10-2) | Free Play |
| The Question | Sewers Interchange (1-2) | Free Play |
| Tim Drake | The Batcave (2-2) | Free Play |
| Trickster | The Containment Facility (4-3) | Free Play |
| Vibe | The Butcher (12-3) | Story |
| Wonder Girl | The Control Room (4-1) | Free Play |
Lego Batman 3: Beyond Gotham eGuide Checklists (Handheld)