The roster of villains in Batman: Arkham Origins continues to grow.

Yesterday, during a panel for the game hosted by creative director Eric Holmes, the latest villain for the upcoming prequel was revealed in a new trailer.  It’s none other than Copperhead, a villain who started out as a he, but is now a female – which means that Harley Quinn isn’t the only lady that Batman has to worry about.

In the trailer, Copperhead makes a daring escape from a prison, only to come face-to-face with the Dark Knight.  Her speed and agility make her a force to be reckoned with – and an assailant who could easily put Batman out of his misery if he’s not careful. 

It was also confirmed that Bane, a long-time antagonist who appeared in last year’s movie The Dark Knight Rises, will also appear in the game.  

Batman: Arkham Origins arrives for Xbox 360, PlayStation 3, Wii U and PC October 25th.